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Image Search Results
Journal: The Journal of general virology
Article Title: Recombinant rotavirus expressing the glycosylated S1 protein of SARS-CoV-2.
doi: 10.1099/jgv.0.001899
Figure Lengend Snippet: Fig. 4. Binding of the SARS-CoV-2 S1 product to the human ACE2 receptor. (a) Whole cell lysates (WCL) were prepared at 9 h p.i. from MA104 cells infected with the indicated rSA11 viruses. The lysates were incubated with hACE2-Fc, a recombinant protein consisting of the extracellular domain of human ACE2 fused to an IgG1 Fc tag. Protein-A spin columns were used to recover hACE2-Fc, and associated proteins, from the lysates. The recovered samples were examined by immunoblot assay using antibodies specific for S1 products (FLAG/His antibody, SARS CoV-2 S1 antibody (ABclonal A20136). The red arrow head identifies S1 protein recovered by the pulldown assay. MWM, molecular weight markers. (b) The residual S1 proteins in the flow through fractions of the protein-A spin columns was determined by immunoblot assay using FLAG/His antibody. The same blot was re-probed with antibodies specific for SARS CoV-2 S1 (αS1/ABclonal), rotavirus VP6, and cellular β-actin.
Article Snippet: Membranes were blocked with phosphate- buffered saline containing 5 % non- fat dry milk and probed with rabbit polyclonal SARS- CoV- 2 S1 antibody (ABclonal A20136, 1 : 1000 dilution),
Techniques: Binding Assay, Infection, Incubation, Recombinant, Western Blot, Molecular Weight
Journal: The Journal of general virology
Article Title: Recombinant rotavirus expressing the glycosylated S1 protein of SARS-CoV-2.
doi: 10.1099/jgv.0.001899
Figure Lengend Snippet: Fig. 5. Localization of SARS CoV-2 S1 protein in rSA11/S1f-infected cells. MA104 cells were mock infected or infected with rSA11/S1f and, at 9 h p.i., fixed with ice cold methanol. Afterwards, the cells were incubated with rabbit S1/ABclonal antibody, mouse NSP2 or NSP4 antibody, followed by Alexa 488 anti-rabbit IgG (green) and Alexa 594 anti-mouse IgG (red) to determine the locations of SARS-CoV-2 S1 and rotavirus NSP2 and NSP4 proteins. Nuclei were detected by staining with DAPI. Cells were analysed with a Nikon Eclipse NiE microscope (100× oil immersion objective) and images were captured with a Hamamatsu Orca-Flash 2.8 sCMOS high resolution camera. Images include 20 µm size bars.
Article Snippet: Membranes were blocked with phosphate- buffered saline containing 5 % non- fat dry milk and probed with rabbit polyclonal SARS- CoV- 2 S1 antibody (ABclonal A20136, 1 : 1000 dilution),
Techniques: Infection, Incubation, Staining, Microscopy